SOPs (Standard Operating Procedures)

Standard Operating Procedures have been issued by VEG-ADAPT to be used for research activity within the project and for the use of stakeholders

1 Screening of tomato, pepper and melon accessions at the pre-flowering stage under combined salt and drought stress and heat stress

2 Calculations of nutrient solution recipes for closed and open hydroponic systems

3 Chlorophyll Assay

4 Optimal conditions for determination of net photosynthetic rate (A), stomatal conductance (GS), intercellular CO2 concentration (CI), transpiration rate (E)

5 Antioxidant assay 1- Ascorbate peroxidase (APX)

6 Antioxidant assay 2- Catalase (CAT)

7 Antioxidant assay 3- Guaiacol peroxidase (G-POD)

8Antioxidant assay 4- Glutathione reductase (GR)

9 Antioxidant assay 5- Superoxide dismutase (SOD)

10 Ferric Reducing Antioxidant Power (FRAP) Protocol

11 Trolox-Equivalent Antioxidant Capacity (TEAC) Assay

12 Total phenolics

13 Total amino acid content

14 Oxidation parameters 1-Malondialdehyde (MDA)

15 Oxidation parameters 2- Hydrogen peroxide (H2O2)

16 Oxidation parameters 3- Electrolyte leakage

17 Bradford protein assay

18 Carbohydrate Assay 1- Sugars (glucose, sucrose, fructose)

19 Carbohydrate assay 2- starch

20 Proline content

21 Total N measurements in tissue and soil with the Kjeldahl method

22 Leaf δ13C discrimination for water use efficiency (WUE) assessment

23 Quality classification of tomato fruits

24 Fruit quality analysis (TSSC, TA)

25Tissue nutrient analysis

26 Soil nutrient analysis

27 Macro- and micro- nutrient analysis in solution

28 Protocol to determine the effects of deficit irrigation on VCC tomato genotypes in greenhouse conditions

29 Protocol to determine the impact of organic amendments and soil cover under deficit irrigation in the hot growing season

30 Protocol to study the combined impact of drought and salt stress in a tomato collection in greenhouse conditions

31 Protocol to analyze transcriptomic responses to drought and heat stress in selected tomato genotypes

32 Protocol to study the impact of drought stress in advanced tomato populations

33 Protocol for the phenotypic characterization of VCC melon genotypes under drought stress in greenhouse conditions

34 Sampling the leaf, root, shoot and the fruit of tomato, pepper, and melon

35 Gas exchange and fluorescence measurements to assess plant performance under water stress conditions and stress treatment application

36 Application of salt stress in experimental crops of tomato, pepper, and melon

37 High-throughput assessment of basic fruit quality traits: ºbrix and acidity

38 Protocol to investigate gene expression via qRT-PCR

39 Protocol for assessment of shoot hydraulic conductivity

40 Evaluation of phenotyping data by calculating Principal Components and Euclidean Distances

EG-ADAPT SOPs are available Open Access in the dedicated Zenodo community at this link